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Fig. 10 | Journal of Neuroinflammation

Fig. 10

From: Intermittent theta-burst stimulation improves motor function by inhibiting neuronal pyroptosis and regulating microglial polarization via TLR4/NFκB/NLRP3 signaling pathway in cerebral ischemic mice

Fig. 10

ITBS modulated microglial phenotypes in the peri-infarcted area. A Immunofluorescence staining for Iba1/CD86 colocalization (Iba1-green and CD86-red) showed the location of M1-polarised microglia (Iba1+/CD86+) among Sham, MCAO/r and iTBS groups. Numerous microglial cells and M1-polarised microglia abundant at the border of infarcted core. Scale bar = 40 μm. B Iba1/CD86 colocalization analysis revealed that iTBS significantly reduced the expression of CD86 on microglia in the peri-infarcted area compared with MCAO/r group. C Immunofluorescence staining for Iba1/CD206 colocalization (Iba1-green and CD206-red) showed the location of M2-polarised microglia (Iba1+/CD206+) among Sham, MCAO/r and iTBS groups. Few CD206 positive fluorescent signal was observed at the border of infarcted core in both MCAO/r and iTBS groups. Scale bar = 40 μm. D Iba1/CD206 colocalization analysis revealed that iTBS significantly up-regulated the expression of CD206 on microglia in the peri-infarcted area compared with MCAO/r group. Values are expressed as the mean and 95% confidence interval (n = 4). *P < 0.05, **P < 0.01 as determined by one-way ANOVA (Tukey’s multiple comparison test) and two-way ANOVA (Bonferroni's multiple comparison test)

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