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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Intermittent theta-burst stimulation improves motor function by inhibiting neuronal pyroptosis and regulating microglial polarization via TLR4/NFκB/NLRP3 signaling pathway in cerebral ischemic mice

Fig. 3

Anti-inflammatory mechanism of iTBS protecting against cerebral ischemia-induced neuronal damage. A Histological images of Nissl staining evaluated the neuronal survival in the ipsilateral cortex. Scale bar = 20 μm. B Quantitative analysis showed that iTBS significantly enhanced survival cells. C TTC staining and D TUNEL staining showed the infarcted size relative to non-ischemic hemisphere after cerebral I/R injury. E Quantitative analysis showed that iTBS significantly reduced infarcted volume. Values are expressed as the mean and 95% confidence interval (n = 4). *P < 0.05, **P < 0.01 as determined by one-way ANOVA (Tukey's multiple comparison test). RNA-seq showed 1157 differentially expressed genes with 42 genes up-regulated, 1115 genes down-regulated (F) and 1297 differentially transcripts with 423 transcripts up-regulated, 874 transcripts down-regulated (G) between MCAO/r and iTBS groups, respectively (n = 3). Volcano map of differentially expressed genes (H) and transcripts (I) between MCAO/r and iTBS groups. Gray represents the genes with insignificant difference. Red and blue represent the genes with significant difference. J GO term enrichment analysis of differentially expressed genes revealed that iTBS protected against cerebral I/R injury possibly by regulating immune system process and innate immune responses

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