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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Intermittent theta-burst stimulation improves motor function by inhibiting neuronal pyroptosis and regulating microglial polarization via TLR4/NFκB/NLRP3 signaling pathway in cerebral ischemic mice

Fig. 4

Spatiotemporal patterns of apoptosis and pyroptosis in the early phase of stroke. A TUNEL staining showed cell apoptosis (green) in the peri-infarcted area before stroke (D0) and at 1 day (D1), 3 days (D3), 5 days (D5), 7 days (D7) after stroke, respectively. Scale bar = 1 mm. (B) Quantitative analysis showed that the apoptosis index robustly increased at D1, and slightly increased from D1 to D7. Quantitative analysis showed that the number of GSDMD positive cells (C) slightly increased and the number of ASC positive cells (D) non-significantly increased between D0 and D1. Subsequently, both the number of GSDMD and ASC positive cells remarkably increased from D1 to D7. Immunofluorescence staining for MAP2/GSDMD (MAP2-red and GSDMD-green) (E) and MAP2/ASC (MAP2-red and ASC-green) (F) colocalization showed the neuronal pyroptosis in peri-infarcted area labeled with MAP2. Scale bar = 40 μm. The spatial patterns between pyroptosis and apoptosis was showed by TUNEL staining (G) and MAP2/GSDMD double staining (H). Values are expressed as the mean ± SEM of the mean (n = 4). Non-significant (ns), *P < 0.05, **P < 0.01 as determined by one-way ANOVA (Tukey’s multiple comparison test)

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