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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Intermittent theta-burst stimulation improves motor function by inhibiting neuronal pyroptosis and regulating microglial polarization via TLR4/NFκB/NLRP3 signaling pathway in cerebral ischemic mice

Fig. 5

ITBS improved local inflammatory status and down-regulated the expression of proteins associated with neuronal pyroptosis. Multiplex cytokine bioassays profiled the levels of 8 available cytokines, including IL-1α (A), IL-1β (B), IL-10 (C), IL-17A (D), IL-27 (E), MCP-1 (F), TNF-α (G) and IFN-γ (H). ITBS significantly ameliorated the high levels of IL-1β, IL-17A, TNF-α, IFN-γ in MCAO/r group and elevated the level of IL-10 (n = 5). Values are expressed as the mean and 95% confidence interval. Representative bands of the western blot (I) and quantitative analysis of pre.Caspase1 (J), cl.Caspase1 (K), pre.IL-1β (L), cl.IL-1β (M), IL-18 (N), ASC (O), GSDMD (P) and NLRP1 (Q) normalized to β-actin (n = 6). ITBS ameliorated cerebral ischemia induced increases in the expression of proteins above. Values are expressed as the mean ± SEM of the mean. Non-significant (ns), *P < 0.05, **P < 0.01 as determined by one-way ANOVA (Tukey’s multiple comparison test)

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Journal of Neuroinflammation

ISSN: 1742-2094