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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Intermittent theta-burst stimulation improves motor function by inhibiting neuronal pyroptosis and regulating microglial polarization via TLR4/NFκB/NLRP3 signaling pathway in cerebral ischemic mice

Fig. 6

ITBS reduced the number of pyroptotic cells and proteins associated with pyroptosis on neurons. A Ultrastructural comparison by electron microscopy among Sham, MCAO/r and iTBS groups (n = 3). Cell membrane pores (Red arrow) formed in ischemic neuron of MCAO/r group, where cell nuclear membrane was complete, but chromatin disappeared. Cell nuclear membrane in iTBS group showed a concave shape, and chromatin condensed (Green arrow). Immunofluorescence staining for NeuN/Caspase1 colocalization (NeuN-red and Caspase1-green) (B), NeuN/GSDMD colocalization (NeuN-red and GSDMD-green) (C) showed the location of pyroptotic cells among Sham, MCAO/r and iTBS groups. Scale bar = 40 μm. Quantitative analysis of cell counts showed that iTBS significantly reduced the number of Caspase1 (D) and GSDMD (F) positive cells in peri-infarcted area. Colocalization analysis revealed that iTBS significantly reduced the expression of Caspase1 (E) and GSDMD (G) on neurons in the peri-infarcted area compared with MCAO/r group. Values are expressed as the mean and 95% confidence interval (n = 4). *P < 0.05, **P < 0.01 as determined by one-way ANOVA (Tukey's multiple comparison test)

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