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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Intermittent theta-burst stimulation improves motor function by inhibiting neuronal pyroptosis and regulating microglial polarization via TLR4/NFκB/NLRP3 signaling pathway in cerebral ischemic mice

Fig. 7

ITBS inhibited expression of ASC in the peri-infarcted area. A Schematic representation of the distribution of necrotic cells, apoptotic/pyroptotic cells and activated glial cells in the different regions of the ischemic hemisphere, including ischemic core and peri-infarcted area. B Immunofluorescence staining for MAP2/ASC colocalization (MAP2-red and ASC-green) showed the location of pyroptotic cells among Sham, MCAO/r and iTBS groups. Scale bar = 40 μm. C Quantitative analysis of MAP2 fluorescence intensity showed that iTBS enhanced the expression of MAP2 in the peri-infarcted area. D ITBS significantly reduced the number of ASC positive cells in peri-infarcted area rather than at the border of infarcted core. E MAP2/ASC colocalization analysis revealed that iTBS significantly reduced the expression of ASC on neurons in the peri-infarcted area compared with MCAO/r group. Values are expressed as the mean and 95% confidence interval (n = 4). Non-significant (ns), *P < 0.05, **P < 0.01 as determined by one-way ANOVA (Tukey’s multiple comparison test) and two-way ANOVA (Bonferroni's multiple comparison test)

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