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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Intermittent theta-burst stimulation improves motor function by inhibiting neuronal pyroptosis and regulating microglial polarization via TLR4/NFκB/NLRP3 signaling pathway in cerebral ischemic mice

Fig. 8

ITBS inhibited expression of GSDMD and NLRP1 in the peri-infarcted area. A Immunofluorescence staining for MAP2/GSDMD colocalization (MAP2-red and GSDMD-green) showed the location of pyroptotic cells among Sham, MCAO/r and iTBS groups. Scale bar = 40 μm. B Quantitative analysis of MAP2 fluorescence intensity showed that iTBS enhanced the expression of MAP2 in the peri-infarcted area. C High number of GSDMD positive cells were located in the peri-infarcted area than that at the border of infarcted core in MCAO/r group, while iTBS significantly reduced the number of GSDMD positive cells in peri-infarcted area. D MAP2/GSDMD colocalization analysis revealed that iTBS significantly reduced the expression of GSDMD on neurons in the peri-infarcted area compared with MCAO/r group. Values are expressed as the mean and 95% confidence interval (n = 4). Non-significant (ns), *P < 0.05, **P < 0.01 as determined by one-way ANOVA (Tukey’s multiple comparison test) and two-way ANOVA (Bonferroni’s multiple comparison test). Immunofluorescence staining for NLRP1/GSDMD colocalization (NLRP1-red and GSDMD-green) (E) and quantitative analysis (F, G) showed iTBS reduced the expression of NLRP1 in GSDMD positive cells in the peri-infarcted area (n = 4). Scale bar = 40 μm. Values are expressed as the mean and 95% confidence interval (n = 4). *P < 0.05, **P < 0.01 as determined by unpaired Student’s t test

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