Fig. 5

Overexpression of Acp5 in PrL pyramidal neurons induced the comorbid-like behavior in naïve rats. A Injective schematic of the AAV-DIO-Acp5-EGFP together with AAV-CaMKIIa-Cre. B Typical images of viral expression within PrL region (Scale bar, 500 µm). C Intra-PrL injection of AAV-Acp5 significantly increased the Acp5 expression in naïve rats (n = 4 or 5 in each group, unpaired t-test, **p = 0.0017 versus the scramble group). D Overexpression of Acp5 in PrL pyramidal neurons increased the immobility time in the FST. (n = 9 or 10 in each group, unpaired t test, ****p < 0.0001 versus the correspondence scramble group). E Distance in central zone and outer zone in open field test were examined following the intra-PrL injection of AAV-Acp5 (n = 10 in each group, unpaired t test, ****p < 0.0001 versus the correspondence scramble group). F Time spent and entries number in the open arms and the closed arms was measured in the EPM test following overexpression of Acp5 in PrL pyramidal neurons. (n = 9 or 10 in each group, unpaired t test, for time, **p = 0.0085 versus the correspondence scramble group; for entries, **p = 0.0086 versus the correspondence scramble group). G Overexpression of Acp5 in PrL pyramidal neurons induced the mechanical allodynia. (n = 9 or 15 in each group, unpaired t-test, ***p < 0.0009 versus the correspondence scramble group). H Number of action potential in PrL pyramidal neurons was decreased following overexpression of Acp5 in PrL pyramidal neurons in naïve rats (n = 20 in each group, Two-way ANOVA, ****p < 0.0001 versus the correspondence scramble group). Data are expressed as mean ± SEM