Fig. 2

FEAMs molecules design rationale and their in-vitro effects on proliferation and aPC activity in N9 microglial cells: A FEAM1-4 structure: the structure of FEAM 1–4 is based on the binding sequence of human aPC/FVII to EPCR. Tosyl group on the N-terminal protects from endopeptidases, and amide modification on the C-terminal contributes to stability. Created with BioRender.com. B Cell proliferation: N9 cells proliferation in the presence of FEAM1-4. In the presence of FEAM2 (green), N9 cells showed significantly lower cell proliferation while, in the presence of FEAM3, N9 cells showed significantly higher cell proliferation, compared to control. N = 24–120. C aPC activity: N9 cells aPC activity was examined in the presence of FEAM1-4. N9 cells showed significantly higher aPC activity in the presence of FEAM1-4, compared to control. N = 23–113. Results are presented as mean ± SEM, *p < 0.05, **p < 0.01, ****p < 0.0001