Fig. 3

Double-labeling immunofluorescence of C3 and S100A10 6 days after TMT treatment. A Co-localization of GFAP and C3 or S100A10 6 days after TMT treatment. B Co-localization of C3 and S100A10 6 days after TMT treatment. Pearson’s correlation coefficient = 0.413 ± 0.0377; Thresholded Mander’s co-localization coefficient (tM) for C3 = 0.523 ± 0.0190; tM for S100A10 = 0.423 ± 0.0123; Costes P-value = 1.000 ± 0.000 (n = 4). Arrows indicate astrocytes that are positive for both C3 and S100A10. Closed arrowheads indicate astrocytes that are positive for C3. Open arrowheads indicate astrocytes that are positive for S100A10. Scale bar = 100 µm