Fig. 6

Effects of RU.521 on STING and related inflammatory factors as well as inflammatory cell infiltration after CVST. Elisa assay and quantitative analysis of 2′3′-cGAMP in diverse groups. n = 6 per group. A Representative western blot images of STING, p-NF-κb p65, NF-κb p65 and MCP-1. n = 6 per group. B Quantitative analysis of western blot assay. ^*P < 0.05, ^**P < 0.01. C Double immunofluorescence staining for STING (green) and microglia (IBA-1, red) in different groups. Scale bar = 50 μm. D Quantitative PCR analysis of cytokines (TNF-α and IL-6) on 3 days post-CVST. n = 5 to 6 per group. ^*P < 0.05, ^**P < 0.01. E Representative images of TNF-α expression in diverse groups. Scale bar = 50 μm. F Flow cytometry assay of brain-infiltrating immune cells including neutrophils (CD11b⁺CD45^highLy6G⁺), monocyte/macrophages (CD11b⁺CD45^highF4/80⁺) and microglia (CD11b⁺CD45^int) in the damaged cortex on 3 days after CVST. G Quantitative analysis of infiltrated immune cells. n = 5 per group. ^*P < 0.05