Fig. 3

Treg increased the expression of CREB and PKC in the brain of TMT-intoxicated mice. Immunohistochemistry was performed for CREB expression in the hippocampi of TMT-intoxicated mice (A). The number of CREB-positive cells among CA1 and CA3 (B) cells was measured (n = 10–13 mice/group). PKC expression was assessed in the hippocampi (C), and the intensity of PKC in CA1 and CA3 (D) was measured using ImageJ software (n = 5–8 mice/group). Data are presented as the mean ± SEM. Significance was determined by Tukey’s HSD and t-test (*p < 0.05, ***p < 0.001 vs. the Con group and ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. the TMT group)