Fig. 4

Treg increased the expression of NeuN and NGF in the brain of TMT-intoxicated mice. Immunohistochemistry was performed for NeuN expression in the hippocampi of TMT-intoxicated AD mice (A). The number of NeuN-positive cells among CA1 and CA3 cells (B) was measured (n = 5–8 mice/group). Immunohistochemistry was performed for NGF expression in the hippocampi of TMT-intoxicated AD mice (C). The intensity of NGF-positive cells in CA1 and CA3 (D) was measured (n = 5–8 mice/group). Data are presented as the mean ± SEM. Significance was determined by Tukey’s HSD (***p < 0.001 vs. the Con group and ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. the TMT group)