Fig. 3

Activity of SCDH superficial laminar neurons following SNS in L5 NRL rats on PID1 and PID7. Transverse sections of the L5 spinal cord were obtained from NRL rats on PID1 (N = 4) and PID7 (N = 5). The border between the superficial (lamina I–III) and deep lamina was set by the heterogenic neuronal size of lamina IV, compared to the smaller cells of lamina III [27], as indicated by the dashed lines (a). Double-immunofluorescence staining of neuronal marker NeuN (green) and c-fos (red) (b). Colocalization of NeuN and c-fos appears yellow in the merged image. Quantification of the number of NeuN/c-fos co-localized cells in a single SCDH section on PID1 (c) and PID7 (d). Scale bar = 200 μm. Data are expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001