Fig. 7

TPH2+ neuronal activity in the RVM following SNS at various frequencies compared to Sham stimulation in L5 NRL rats on PID1. Transverse sections of rostral brainstem were obtained from NRL rats on PID1 (a) (N = 3). Magnification of the RMg (A, right panel). Double-immunofluorescence staining of the serotoninergic neuron marker TPH2 (green) and c-fos (red) (b). Quantification of the number of TPH2/c-fos co-localized cells (c). Scale bar = 50 μm. Data are expressed as mean ± SD.*p < 0.05, **p < 0.01