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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: FKBP51 mediates resilience to inflammation-induced anxiety through regulation of glutamic acid decarboxylase 65 expression in mouse hippocampus

Fig. 1

Fkbp5 deletion increases sensitivity to LPS-induced anxiety responses. C57BL/6J (WT) and Fkbp5-KO mice were subjected to a single intraperitoneal injection of saline (SAL) or LPS (3 mg/kg of body weight). A, B The mean body weight (n = 7 mice per group) and food intake (n = 5 mice per group) of mice were tracked every day for 7 days to assess sickness behavior. Data are expressed as the mean ± SEM. ***p < 0.001 for the comparison between two groups in a two-way ANOVA. C The sucrose preference test was performed to assess the anhedonia-like phenotype. Representative traces (D) and the time spent in the central zone (E) of the open-field test (OFT). Spontaneous locomotor activity was expressed as the total distance traveled in the OFT (F). Representative traces (G), time spent in the open arms (H), and total distance traveled (I) in the elevated plus maze (EPM). n = 7 mice per group. J, K qRT-PCR analysis of both hippocampal (HPC) and liver Fkbp5 expression at days 1 and 7 after SAL or LPS injection (n = 5 mice per group). *p < 0.05, **p < 0.01, and ***p < 0.001 for the comparison between two groups in a two-way ANOVA followed by the Tukey post hoc test. L Western blotting indicated that LPS increased FKBP51 protein expression at day 7 in the HPC (n = 5 mice per group). The relative band intensities were normalized to GAPDH levels. *p < 0.05 for the comparison between two groups in an unpaired Student’s t test

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