Fig. 2

Fkbp5 deletion attenuates LPS-induced hippocampal immune responses. A, B Spleens were dissected and weighed on day 1 and 7 post-injection of SAL or LPS (n = 6 mice per group). Scale bar: 1 cm. C Complete blood count analysis of peripheral blood samples collected from mice 1 or 7 days after saline or LPS injection. The monocyte counts are shown (D1, n = 3 mice per group; D7, n = 6 mice per group). D–F The liver and HPC of WT and Fkbp5-KO mice were analyzed by qRT-PCR for liver Tnf-α (D), HPC Tnf-α (E), and HPC Il-6 (F) mRNA. Note that Tnf-α expression was induced on day 1 in both the liver and HPC, whereas delayed induction of Il-6 in the HPC was observed on day 7. Data are indicated as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 for the comparison between two groups in a two-way ANOVA followed by the Tukey post hoc test. G Representative immunofluorescence images of microglial marker Iba-1 (red) and DAPI (blue) in the mouse hippocampal dorsal and ventral CA1 (dCA1 and vCA1). Scale bar: 100 μm. H, I Quantification of the area expressing Iba-1 in the stratum pyramidale (SP) and stratum radiatum (SR) of the hippocampal CA1 region (D1, n = 3 mice per group; D7, n = 5 mice per group). Data are indicated as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 for the comparison between two groups in an unpaired Student’s t test