Fig. 6

Transplantation of iPSCs regulated microglia polarization and alleviated neuroinflammation. A, B Microglia polarization were determined by immunofluorescence staining, TMEM119 was used to label microglia (red), and iNOS (blue) for M1 or Arg1 (blue) for M2 in samples collected at 14 dpi. after injury. Scale bar = 60 µm. C Relative expression of pro-inflammatory (IL-1β and TNF-α) and anti-inflammatory (IL-4 and IL-10) cytokines detected by qRT-PCR at 14 d post-injury (*p < 0.05, **p < 0.01, ***p < 0.001 vs. SCI group; ^##p < 0.01 GFP vs. H-Apln, mean ± SD)