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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Anti-IgLON5 antibodies cause progressive behavioral and neuropathological changes in mice

Fig. 4

Anti-IgLON5 IgG bind in vivo for a long time and cause damage to neurons in the hippocampus. A Representative images of IgG binding to the hippocampus in perfused fixed brains on day 7. Scale bar = 100 µm. B Representative images of IgG binding to the hippocampus in perfused fixed brains on day 30. Scale bars = 100 µm. C Quantification of the intensity of human IgG immunolabeling in the hippocampus of mice injected with patient IgG or control IgG killed on day 7 (total: t = 10.27, df = 6, p < 0.0001; unpaired t-test; CA1: t = 0.0007, df = 6, p = 0.0007; unpaired t-test; CA3: t = 4.691, df = 6, p = 0.0034; unpaired t-test; DG: t = 8.742, df = 6, p = 0.0001; unpaired t-test). D Quantification of the intensity of human IgG immunolabeling in the hippocampus of mice infused with patient IgG or control IgG killed on day 30 (total: t = 6.280, df = 8, p = 0.0002; unpaired t-test; CA1: t = 7.678, df = 8, p < 0.0001; unpaired t-test; CA3: t = 0.6918, df = 8, p = 0.5086; unpaired t-test; DG: t = 3.885, df = 8, p = 0.0046; unpaired t-test). E Representative pictures of NeuN expression in the hippocampus of mice injected with patient IgG or control IgG killed on day 7. Scale bars = 20 μm. The white arrows on the left and right represent the same neuron. F Quantification of NeuN-positive cell density in the total hippocampus and CA1 region on day 7. G Representative pictures of NeuN expression in the hippocampus of mice on day 30. Scale bars = 20 μm. The white arrows on the left and right represent the same neuron. H Quantification of NeuN-positive cell density in the total hippocampus and CA1 region on day 30 (t = 2.935, df = 6, p = 0.0261; unpaired t-test). I Representative pictures of Nissl-stained sections in the hippocampus of mice injected with patient IgG and control IgG and killed on day 30. Scale bars = 100 μm. J Quantification of Nissl-stained positive cell densities in the total hippocampus and CA1 regions on day 30 (CA1: t = 2.705, df = 13, p = 0.018; unpaired t-test; Total: t = 0.4548, df = 13, p = 0.6568; unpaired t-test). For A–D, n = 4 or 5 per group. For (E)–(H), n = 4 or 5 per group. For I–J, n = 7 per group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs control IgG group. NeuN = neuronal nuclear antigen

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