Fig. 3
FIR light did not influence the Aβ production but enhanced microglial Aβ phagocytosis. A The Western blot analysis of key molecules involved in the process of Aβ production. Expression level of B APPfl, C sAPPα, D Nicastrin, E BACE1 and F PS1 (n = 5 per group). G Representative images of Aβ (6E10, red), microglia (Iba1, green) and nuclei (DAPI, blue) co-staining from APP/PS1 treated with or without FIR light. H Quantification of microglial cells within 20 μm from the Aβ plaque boundary (n = 102 to 103 plaques per group) in the cerebral cortex. I Representative images of Aβ (6E10, white), microglia (Iba1, red), phagosome (CD68, green) and nuclei (DAPI, blue) co-staining in the cerebral cortex of APP/PS1 mice treated with or without FIR light. J Quantification of the percentage of phagosome area in Iba1 positive area (n = 49–52 per group). K Quantification of the percentage of 6E10+/CD68+/Iba1+ co-staining area normalized to the total 6E10+ area (n = 49–52 per group). L Representative images of the uptake of FAM-Aβ1-42. M Image quantification of the corresponding fluorescent intensity of FAM-Aβ1-42 engulfed by microglia (n = 6 per group). Data were means ± SEM, ***p < 0.001. N.S., not significant. CytoD: cytochalasin D