Fig. 7

ABX treatment reduces STZ-induced activation of microglia and astrocyte in the spinal cord of mice. A Representative images of the Iba-1 microglia immunostaining (scale bar: 100 µm) and the quantification of the fluorescence intensity (upper) and cell numbers (lower) of the Iba1 immunostaining cells. One-way ANOVA with Dunnett multiple comparison test **p < 0.01 versus SPF, ^##p < 0.01 versus SPF + STZ (15 slides from 4 SPF and ABX mice, 28 slides from 4 mice with SPF + STZ, and 24 slides from 4 mice with ABX + STZ). B Representative images of the GFAP astrocyte immunostaining (scale bar: 100 µm) and the quantification of the fluorescence intensity of the GFAP immunostaining cells. One-way ANOVA with Dunnett multiple comparison test **p < 0.01 versus SPF, ^#p < 0.05 versus SPF + STZ (21 slides from 4 SPF mice, 24 slides from 4 ABX mice, 22 slides from 4 SPF + STZ mice, and 23 slides from 4 ABX + STZ mice). Tissues were taken from SPF and ABX-treated mice at day 42 after STZ or sham operation (A and B)