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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Positive interaction between GPER and β-alanine in the dorsal root ganglion uncovers potential mechanisms: mediating continuous neuronal sensitization and neuroinflammation responses in neuropathic pain

Fig. 2

GPER not ERα or ERβ, contributes to spared nerve injury (SNI)-induced pain behaviours in the dorsal root ganglion (DRG) but not in the spinal dorsal horn (SDH). A Quantification of fluorescence intensity showed the G protein-coupled oestrogen receptor, oestrogen receptor-α and oestrogen receptor-β expression in DRG and dorsal spinal cord of SNI rats compared to sham rats (n = 6–7 sections from three rats). Two-tailed unpaired Student’s t test.***p < 0.001, **p < 0.01 versus Sham. Scale bars = 20 μm (DRG) and 10 μm (SDH). D, E Intrathecal injection of the G protein-coupled oestrogen receptor-1 agonist G1 (0.2 μg/μl, 1 μl), the oestrogen receptor-α agonist PPT (0.02 ng/μl, 1 μl) and the oestrogen receptor-β agonist DPN (0.02 ng/μl, 1 μl), directly into the subarachnoid space induced mechanical allodynia and cold hyperalgesia. Kruskal–Wallis test followed by Dunn’s multiple comparison test. ***p < 0.001 versus vehicle (10% dimethyl sulfoxide); n = 8 for each group

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Journal of Neuroinflammation

ISSN: 1742-2094