Fig. 8

Microinjection of anti-CX3CL1 antibody into ACC attenuates SNI-induced mechanical allodynia and inhibits ACC TNF-α and Nav1.6 protein upregulations. A, B Behavioral test paradigms are shown on the first left, and the ipsilateral and contralateral test results are shown on the second and first right, respectively. Statistical significance was determined by Dunn's multiple comparisons test (*p < 0.05; **p < 0.01 versus PO -1d) or multiple t tests (#p < 0.05 versus IgG control). C The mark left by the cannula implantation showing the drug injection site in Cg1 region. D Representative western blotting of CX3CL1, TNF-α and Nav1.6 in bilateral ACC following contralateral administration of anti-CX3CL1 antibody (1 μg/μl, 3 μl) 7d post-SNI. E The quantification results showing the inhibitory effects of anti-CX3CL1 antibody on SNI-induced CX3CL1, TNF-α and Nav1.6 expressions. *p < 0.05, **p < 0.01, ***p < 0.001 versus IgG control (one-way ANOVA)