Fig. 1

cIAP2 deficiency exacerbates EAE disease. A Mean clinical EAE scores in WT (n = 11) and cIAP2^−/− (n = 10) mice. Data are representative of two independent experiments. B Disease onset, peak symptoms, and percentage of mortality for WT (n = 11) and cIAP2^−/− (n = 10) mice. Inflammatory cytokine (C) and chemokine (D) mRNA expression in naïve (n = 4, 4) and EAE (n = 5, 5) lumbar spinal cords (day 12). E H&E staining of lumbar spinal cords (L2-L4) collected at day 12 of disease (representative images). E’ Quantification of inflammatory cells infiltrating lumbar spinal cords (L2-L4) (6 areas each). F T cell infiltration. Flow cytometry gating of CD4⁺ and CD8⁺ T cells (initially gated as CD45⁺CD11⁻ lymphocytes) determined at day 12 of EAE in the WT (n = 4) and cIAP2^−/− (n = 4) brains. G Luxol Fast Blue (Quantification; 5 areas each), Fluoromyelin (H) (Quantification; 50 areas each), and GFAP I (Quantification; 15 areas each), staining of lumbar spinal cords (L2-L4) collected at day 12 of disease (representative images). A–D, F Mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, T-test