Fig. 3

Surgery caused a selective reduction in the levels of synaptic NR2A and NR2B subunits. a Representative immunoblots and densitometry analysis of b NR2A, c NR2B, and d NR1 proteins levels in the dHPC on postoperative days 1, 6, 13, 20, and 59 (n = 6 rats per group). e Representative immunoblots and f densitometry analysis of NR2A, NR2B, NR1, GluA1, and GluA2 proteins levels in the dHPC on postoperative day 13 (n = 6 rats per group). g Representative immunoblots showing levels of the NR2A, NR2B, and NR1 proteins in the total dHPC H, SM, and SPM. h Densitometry analysis of NR2A, NR2B, and NR1 proteins levels in the SM and SPM (n = 6 rats per group). Representative immunoblots showing levels of i the PSD-95, Synaptophysin, and j Calnexin indicated the efficiency of separating TxP (synaptic) and TxS (extrasynaptic) fractions of the dHPC. j Representative immunoblots and densitometry analysis of (k) NR2A, l NR2B, and m NR1 proteins levels in the TxS and TxP fractions of the dHPC on postoperative day 13 (n = 6 rats per group). n Representative confocal images of double staining for NR2A (green puncta) at the extrasynaptic (colocalized with synaptophysin⁺ red puncta) and synaptic (colocalized with PSD-95⁺ red puncta) sites (left panel) and NR2B (blue puncta) at the extrasynaptic (colocalized with synaptophysin⁺ red puncta) and synaptic (colocalized with PSD-95⁺ red puncta) sites (right panel) captured with a 63 × objective. C control, S surgery, H homogenate, SM synaptosomal membrane, SPM synaptic plasma membrane, SYP Synaptophysin, TxS Triton X-100-soluble, TxP Triton X-100-insoluble. Data are presented as means ± SEM and statistical analyzed by a two-way ANOVA followed by a Tukey’s test (b, c, and d), a Student’s t test (f, h, k, l, and m). *p < 0.05, **p < 0.01, and ***p < 0.001 compared with Control