Fig. 10

NKCC1-mediated transmembrane cotransport of ions and water after NLRP3 activation. A Representative images of Na⁺ (SBFI) and K⁺ (PBFI) after LPS or lysis-RBC exposure and MCC950 treatment in primary choroid plexus epithelial cells. Bar = 50 μm. B Statistical results of Na⁺ concentrations in primary choroid plexus epithelial cells according to SBFI staining (n = 6, one-way ANOVA). C Statistical results of K⁺ concentrations in primary choroid plexus epithelial cells according to PBFI staining (n = 6, one-way ANOVA). D Time course of Na⁺ concentration changes in primary choroid plexus epithelial cells after the addition of stimulators and MCC950 treatment (n = 6). E Changes in intracellular K⁺ concentrations over time after different treatments (n = 6). F–G Na⁺ and K⁺ concentrations measured by ICP-OES in culture media after LPS and lysis-RBC stimulation and MCC950 treatment (n = 6, one-way ANOVA). The values are expressed as the means ± SDs; **p < 0.01 and *p < 0.05