Fig. 8

Effects of bumetanide treatment on the choroid plexus after ICH-IVH. A, B Representative images of immunofluorescence staining for p-NKCC1 (A) and p-NKCC1-positive cell statistics (B) of the choroid plexus at 3 days following bumetanide treatment (6 rats/group, Welch’s two-tailed unpaired t-test). Bar = 50 μm. C CSF secretion rates after bumetanide treatment (6–8 rats/group, Welch’s two-tailed unpaired t-test). C–E Western blot analysis of p-NKCC1 and NLRP3 in the choroid plexus at 3 days after bumetanide treatment (n = 5, 6 rats/sample, Welch’s two-tailed unpaired t-test). F CSF secretion rates after bumetanide treatment (6–8 rats/group, Welch’s two-tailed unpaired t-test). The values are expressed as the means ± SDs; **p < 0.01; ns, p > 0.05