Fig. 5

Nafamostat inhibited the expression of thrombin, PAR1 and MMP9 in endothelial cells. A Immunofluorescence image of thrombin (red) and CD31 (green) at 3 dpi. Cell nuclei were stained with DAPI (blue). Scale bar = 100 μm, 25 μm. B Quantitative analysis of the relative fluorescence intensity of thrombin (data shown as mean ± SEM, one-way ANOVA with Tukey's post hoc test, ***P < 0.001, n = 3). C Immunofluorescence image of CD31 (red) and PAR1 (green). Cell nuclei were stained with DAPI (blue). Scale bar = 25 μm. D Quantitative analysis of the relative fluorescence intensity of PAR1 (data shown as mean ± SEM, one-way ANOVA with Tukey's post hoc test, *P < 0.05, **P < 0.01, n = 3). E Immunofluorescence image of CD31 (red) and MMP9 (green). Cell nuclei were stained with DAPI (blue). Scale bar = 10 μm. F Quantitative analysis of the relative fluorescence intensity of MMP9 (data shown as mean ± SEM, one-way ANOVA with Tukey's post hoc test, ***P < 0.001, n = 3). G The schematic diagram describes the location of the fluorescent photo. H Model diagram of the effect pathway of nafamostat on ECs after SCI