Fig. 1

Repopulation of microglia does not ameliorate Aβ pathology and neuritic damage in male 3xTg mice. Experimental paradigm depicting duration of PLX5622 treatment and subsequent microglial repopulation (A). Representative confocal immunofluorescent 20× images of the subiculum in control versus PLX-repopulated group, showing Aβ plaque (6E10, red), microglia (Iba1, magenta), neuritic damage (LAMP1, green) (B). Scale bar represents 200 µm. There were no significant differences in the total area (C, G), size (D, H) and number (E, I) of plaques between the control and PLX-repopulated group in both cohorts. Ratio of plaque-associated LAMP1⁺ neuritic damage to plaque load was similar between the control and PLX-repopulated groups in both cohorts (F, J). Representative confocal immunofluorescent 20× images of the subiculum in control versus PLX-repopulated group, showing Aβ plaque labeled with MeX04 (blue) and 6E10 (red) (K). There was no difference in the total plaque area labeled with MeX04 (L) and the ratio of MeX04/6E10 (M) between the two treatment groups. Student’s t-test. Data are presented as mean ± SEM (Cohort 1: n = 9–11; Cohort 2: n = 7)