Fig. 5

dBET1 reduces ischemia-induced blood–brain barrier breakdown. A Representative images of IgG stain in the post-stroke mouse brain sections, showing the leakage of serum immunoglobulin G (IgG) through the disrupted blood–brain barrier (BBB) at 48 h after tMCAO; and open squares in the left image indicate the peri-infarct areas of the ipsilateral (Ipsil) cortex and striatum, as well as the contralateral (Ctrl) side, used for micrographic examination. No IgG stain signal is observed in the contralateral side of mouse brains. Apparent IgG stains are widely detected in the peri-infarct area, which is dramatically reduced in the dBET1 group. B Quantifications of IgG signals in A showed that dBET1 significantly reduced the IgG extravasation compared to veh controls. n = 6 per group. *P < 0.05, **P < 0.01. C Representative immunoblots for tight junction proteins ZO-1 and occludin, two important structural components of the BBB, in homogenates from the ischemic cortex and sham controls. β-actin was used as a loading control. D Quantitative analysis shows that stroke resulted in marked degradation of ZO-1 and occludin in the ischemic cortex than sham controls. In contrast, such degradation of these two proteins was significantly attenuated in the dBET1 stroke group. n = 5 per sham group, n = 6 per stroke group. *P < 0.05, **P < 0.01, ***P < 0.001