Fig. 6

CXCR2 enhances monocyte-mediated ICD of nerve cells. A Typical apoptosis images of SH-SY5Y cells co-cultured with TBI/non-TBI serum-educated monocytes, and with DMSO (vehicle control) or SB225002 treatment. B Percentage of apoptotic SH-SY5Y cells, co-cultured with TBI/non-TBI serum-educated monocytes and with DMSO (vehicle control) or SB225002 treatment. *P < 0.05, **P < 0.01, determined by paired t tests. C Typical immunofluorescence images of HMGB-1 expression in SH-SY5Y cells co-cultured with TBI serum-educated monocytes and with DMSO (vehicle control) or SB225002 treatment. D HMGB-1 levels in the supernatant of SH-SY5Y cells alone (control), or co-cultured with TBI serum-educated monocytes and with DMSO (vehicle control) or SB225002 treatment. **P < 0.01, determined by paired t tests. E Typical flow cytometry results of surface calreticulin (CRT) expression on SH-SY5Y cells alone (control), or co-cultured with TBI/non-TBI serum-educated monocytes and with DMSO (vehicle control) or SB225002 treatment. F Percentage of CRT⁺ cells in SH-SY5Y cells alone (control), or co-cultured with TBI/non-TBI serum-educated monocytes and with DMSO (vehicle control) or SB225002 treatment. **P < 0.01, determined by paired t tests. G Extracellular ATP levels in the supernatant of SH-SY5Y cells alone (control), or co-cultured with TBI/non-TBI serum-educated monocytes and with DMSO (vehicle control) or SB225002 treatment. *P < 0.05, **P < 0.01, determined by paired t tests. Data are presented as mean with SEM