Fig. 6

S-2HG alters CD8⁺ T cell proliferation and activation. A Representative immunostaining of CD8⁺ T cells treated with vehicle, 200 μM S-2HG, 500 μM S-2HG, empty vector, L2ghdh-Flag, shScramble, shL2hgdh followed by Edu pulse labeling. Scale bar = 10 μm. B–D Percentage of Edu-positive cells in CD8⁺ T cells treated with S-2HG (B), L2ghdh-Flag (C), or shL2hgdh (D). E–G Flow cytometry analysis on CD44^hiCD62L^lo percentage on CD8⁺ T cells treated with S-2HG (E), L2ghdh-Flag (F), and shL2hgdh (G). *P < 0.05, **P < 0.01, ns indicates nonsignificant