Fig. 2

Drp1/Fis1 axis accelerates mitochondrial dysfunction in the cerebral cortex of CKD mice. A Heat map of expression of upregulated genes in RNA-Seq. B Venn map of intersection between upregulated genes in RNA-Seq and “Renal Failure”-related genes in GeneCards and DisGeNET. C The genes interacted with Fis1 predicted by STRING. The redder the circle where the gene located indicates the higher combined_score. Drp1 (DNM1L) has the highest combined_score. D FIS1 and Drp1 expression in RNA-Seq cerebral cortex sample of normal mice (blue, n = 3) and CKD mice (red, n = 3). E mRNA levels of Fis1 and Drp1 in the cerebral cortex of CKD mice determined by RT-qPCR. F Expression of Fis1 and Drp1 proteins in the cerebral cortex of CKD mice detected by immunohistochemical staining. G–J MDA level (G), SOD activity (H), ATP level (I), and ROS level (J) in the cerebral cortex of CKD mice. K The morphology of mitochondria in cerebral cortex of CKD mice observed under the TEM (scale bar = 1 μm) and mean mitochondria size. N = 6. *p < 0.05