Fig. 4

Promotive effect of Drp1 on EZH2 promoter activity and expression. A EZH2 expression in RNA-Seq cerebral cortex sample of normal mice (blue, n = 3) and CKD mice (red, n = 3). B EZH2 mRNA level in prefrontal cortical tissues of CKD mice determined by RT-qPCR. C EZH2 protein level in prefrontal cortical tissues of CKD mice measured by Western blot analysis. CKD mice were treated with calcitonin alone or combined with overexpressed Drp1 (n = 6). D EZH2 mRNA level in prefrontal cortical tissues of CKD mice determined by RT-qPCR. E Protein levels of Drp1 and EZH2 prefrontal cortical tissues of CKD mice measured by Western blot analysis. Mouse primary cortical neurons were transduced with lentivirus overexpressing Drp1. F mRNA levels of Drp1 and EZH2 in mouse primary cortical neurons determined by RT-qPCR. G EZH2 promoter activity detected by dual-luciferase reporter gene assay. N = 6 for mice, and n = 3 for cells. *p < 0.05