Fig. 7

The effect of C5a on Smad2/3 and ERK expression in RPE cells. Primary mouse RPE cells were treated with C5a (50 ng/mL) for 24 h with or without PMX53 (50 nM). The expression of pSmad2/3, ERK, and p90RSK was determined by Western blot. A Representative Western blot images of pSmad2/3. B Quantification of pSmad2/3 expression in different groups. C Representative Western blot images of ERK1/2 and p90RSK. The pERK antibody recognizes pERK1 (Y204) + pERK2 (Y197). D Quantification of pERK1/2 expression in different groups. E Quantification of p90RSK (S380) expression in different groups. Mean ± SEM, n = 4–6 (from 2 ~ 3 independent studies). *p < 0.05; **p < 0.01; ***p < 0.005, ANOVA one-way followed by Tukey’s post hoc test