Skip to main content
Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells

Fig. 8

C5aR expression in the subretinal fibrotic lesion in mouse eyes. A Representative image of normal mouse eye section showing C5aR (red) expression in RPE cells (arrowheads) and choroidal cells (arrows). B Representative image of a RPE/choroid flatmount from day 10 post the second laser mouse eye stained for Collagen-I (Col-1, green), C5aR (red) and DAPI. C A confocal image showing Col-1 (red), MHC-II (green) and C5aR (blue). Arrows indicate MHC-II+C5aR+ cells. D A confocal image of mouse eye section 20 days after the second laser stained for fibronectin (Fn, green), C5aR (red) and DAPI showing C5aR (red) expression in subretinal fibrotic lesion. E Isotype control staining of a RPE/choroidal flatmount showing no immunoreactivity of collagen-1 and C5aR. Scale bar = 50 µm. Col: collagen-1; Fn: fibronectin; Ch: choroid; dRPE: damaged retinal pigment epithelial layer; Fibr: fibrotic lesion; Re: retina

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094