Fig. 9

Effect of C5aR blockade on a mouse model of subretinal fibrosis. Subretinal fibrosis was induced in C57BL/6J mice using the two-stage laser-induced protocol (see Methods). A Schematic diagram shows experimental design of the in vivo C5aR blockade study. PMX53 (1 mg/kg) was administered subcutaneously daily from day 0 until day 9. On day 10, mice were subjected to fundus imaging and fluorescence angiography before killing the animals. B, C Representative fundus colour photography (B) and fluorescence angiography (C) in PMX53 and vehicle-treated (control) mice at day 10 post second laser. (E) Quantitative measurement of the area of fluorescein leakage from fibrosis lesion (in pixels). Mean ± SEM, n = 24–30 lesions from 8–9 eyes/group. *p < 0.005, Mann–Whitney test. D Representative images of Col-I⁺ lesions in RPE/choroid flatmount of vehicle and PMX53-treated mice. Scale bar = 100 μm. F Quantitative measurement of collagen-I⁺ lesion area in vehicle and PMX53-treated mice. Mean ± SEM, n = 30 lesions from 8 eyes