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Table 1 Recombinant proteins and primary antibodies used in the study

From: Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells

Recombinants/molecules

Product name

Cat. No.

Company

Recombinant Mouse Complement C3a, CF

8085-C3

R&D Systems

Recombinant Mouse Complement C5a

2150-C5-025

R&D Systems

Recombinant Mouse TGF-β2

7346-B2-005

R&D Systems

PMX 53 (C5a receptor antagonist)

Cat. No. 5473

Tocris-Biotechne

Antibodies

Product name

Cat. No.

Company

Host

Fibronectin

ab2413

Abcam

Rabbit

E Cadherin antibody

orb213706

Biorbyt

Rabbit

α-SMA (-Cy3 conjugated)

C6198

Sigma-Aldrich

Mouse

Rabbit anti-α-SMA

ab5694

Abcam

Rabbit

C5b-9

ab65811

Abcam

Rabbit

C5aR

ab59390

Abcam

Rabbit

C5aR

ab117579

Abcam

Rat

C5R1 Biotin

ab54378-100

Abcam

Biotin

C5a antibody (FITC)

orb360860

Biorbyt

Rat

C5a antibody

MAB21501-100

R&D Systems

Goat

Collagen 1 Rabbit pAb

ab34710

Abcam

Rabbit

Gt X Collagen Type I

AB758

CHEMICON

Goat

Vimentin polyclonal antibody

Orb304659

Biorbyt

Rabbit

Slug antibody

PSI-3957

ProSci

Rabbit

MHC Class II (I-A/I-E) Monoclonal Antibody

14–5321-82

eBioscience

Rat

GAPDH

G8795

Sigma

Mouse

AKT/MAPK Pathway Antibody Cocktail

ab151279

Abcam

Rabbit

pSmad2/3

8828

Cell Signaling Technologies

Rabbit