Fig. 8

Results of luciferase reporter experiments. A Prediction of binding effect between miR-22-3p and Sox9 on the RNAhybrid site. B The luciferase assay shows that miR-22-3p causes a decrease in fluorescence in the wild group but not in the mutant group. C Sox9 expression is increased in AD brain tissue based on the GEO database. D Results of GSEA shows that miR-22-3p binds to more genes in the group with high expression of Sox9. E The expressions of Sox9 and NF-kB proteins are decreased as the level of miR-22-3p is elevated. F Statistics of western blot results. *P < 0.05, ***P < 0.005, n = 3