Fig. 10

Nilotinib reduces LPS-induced p-P38 and p-STAT3 protein levels in wild-type mice. a, c Immunofluorescence staining of p-P38 and p-STAT3 in brain slices from wild-type mice injected with vehicle (5% DMSO, 10% PEG and 20% Tween 80 in deionized water) or nilotinib (20 mg/kg, i.p.) daily for 7 days followed by LPS (10 mg/kg, i.p.) or PBS on day 7. b, d Quantification of data in a and c (p-P38, n = 14–24 brain slices from 3 to 4 mice/group, p-STAT3, n = 21–24 brain slices from 3 to 4 mice/group). e, f Western blotting analysis of p-P38 and p-STAT3 in the cortex and hippocampus in wild-type mice (n = 4/group). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar = 100 μM