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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Mesenchymal stromal cell treatment attenuates repetitive mild traumatic brain injury-induced persistent cognitive deficits via suppressing ferroptosis

Fig. 2

The occurrence of ferroptosis was detected after rmTBI. A, B Western blot was performed to observe the time course of changes in the expression of iron metabolism-related proteins (Tfr1 and Fpn). Protein expression was normalized to GAPDH and expressed as fold change of sham. C, D The time course of changes in Fe2+ content and GPx activity. E Representative immunofluorescence staining of GPx4-positive cells in the cortex and hippocampus at different time points after rmTBI and quantitative analysis of GPx4-positive cells. Red indicates positive GPx4 staining, and blue indicates positive DAPI nuclear staining. Scale bars = 100 μm. F, G Western blot was performed to observe the time course of changes in the expression of 4-HNE and GPx4. Protein expression was normalized to GAPDH and expressed as fold change of sham. H The ultrastructure of the cortex on day 28 after rmTBI in mice was captured by transmission electron microscopy. The white arrow indicates mitochondria. I Histogram showing the frequency of mitochondrial area in the cortex. Number of mitochondria, sham: n = 101; rmTBI 28 d: n = 128. Scale bars = 1.0 μm. Data are expressed as mean ± SD (n = 6) and analyzed using one-way ANOVA with Dunnett’s post hoc test (B-E, G). *P < 0.05, **P < 0.01, and ***P < 0.001 versus sham

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