Fig. 6

MSCs treatment rescued rmTBI-induced neurodegeneration and neuronal loss. A Representative immunofluorescence staining of FJC-positive cells in the cortex and hippocampus of sham, vehicle, MSCs, and Lip-1 groups. Green indicates FJC-positive staining, and blue indicates positive DAPI nuclear staining. Scale bar = 25 μm. B Quantitative analysis of FJC-positive cells. C, D Western blot analysis of NeuN proteins in the sham, vehicle, MSCs, Lip-1 groups. Protein expression was normalized to β-actin and expressed as a fold change of sham. Data are expressed as mean ± SD (n = 6) and analyzed using one-way ANOVA with Tukey’s post hoc test. For vehicle group: **P < 0.01, and ***P < 0.001 vs sham group; for MSCs group: ^##P < 0.01, and ^###P < 0.001 vs vehicle group; for Lip-1 group: ^#P < 0.05, ^###P < 0.001 vs vehicle group