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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: NOX2-mediated reactive oxygen species are double-edged swords in focal cerebral ischemia in mice

Fig. 2

Dynamic changes in NOX2 and ROS during stroke. A, C The dynamic production of ROS was revealed by DHE staining and was closely dependent on the expression of NOX2, and the change in ROS was similar to the change in NOX2. Apocynin significantly inhibited ischemia/reperfusion-induced increases in ROS levels. B, D Representative western blots and quantitative analysis of the levels of NOX2 and the effects of apocynin on NOX2 expression in ischemic cortical tissue at the indicated time points during reperfusion. The expression of NOX2 was increased 3 days poststroke but gradually decreased to a level similar to that in the sham group over 14 days. Apocynin (2.0 mg/kg intraperitoneal injection, i.p.) markedly decreased the expression of NOX2 in ischemic cortical tissue. *P < 0.05 vs. the vehicle group, &P < 0.05 vs. the sham group, #P < 0.05 vs. the 3d MCAO group, ^P < 0.05 vs. the 7d MCAO group. Scale bar = 50 µm. All data are expressed as the mean ± SD and were analyzed by one-way ANOVA with Bonferroni's post hoc test. There were 5 animals in each group

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