Fig. 5

Dynamic changes in autophagy-related proteins during stroke. A, C Expression of LC3B was revealed by immunofluorescent staining and was increased on Day 3 and gradually decreased with increasing time, and the expression of LC3B was inhibited by apocynin in all groups, which had a similar trend with NOX2 and ROS (Fig. 2). B Representative western blots and quantitative analysis of the levels of Beclin-1 and p62 and the effects of apocynin on the expression of these factors in ischemic cortical tissue at the indicated timepoints during reperfusion. D Quantitative analysis of Beclin-1 levels. The expression of Beclin-1 was increased 3 days poststroke but gradually decreased over time until 14 days poststroke. Apocynin downregulated Beclin-1 expression in all groups, accompanied by NOX2 and ROS decreases. E Quantitative analysis of levels of p62. The levels of p62 were reduced 3 days after MCAO but gradually increased with time until 14 days. Apocynin upregulated p62 expression in all groups, accompanied by NOX2 and ROS decreases. Scale bar = 50 µm. *P < 0.05 vs. the vehicle group, ^&P < 0.05 vs. the sham group, ^#P < 0.05 vs. the 3d vehicle group, ^{^}P < 0.05 vs. the 7d vehicle group. All data are expressed as the mean ± SD and were analyzed by one-way ANOVA with Bonferroni’s post hoc test. There were 5 animals in each group