Fig. 7
From: NOX2-mediated reactive oxygen species are double-edged swords in focal cerebral ischemia in mice
Apocynin increased the expression of NLRP3 inflammasome-associated proteins via autophagy 7 days after stroke. A Representative western blot showing that rapamycin inactivated, while 3-MA promoted, the expression of NLRP3-associated proteins, including NLRP3, ASC, CL-caspase-1, HMGB1 and IL-1β, in ischemic cortical tissue after 7 days of reperfusion. Apocynin plus 3-MA or rapamycin increased or maintained the expression of NLRP3 inflammasome-associated proteins. B–F Quantitative analysis of the protein levels of NLRP3, ASC, CL-caspase-1, HMGB1 and IL-1β in ischemic cortical tissue. *P < 0.05 vs. the sham group, &P < 0.05 vs. the vehicle group, #P < 0.05 vs. the APO group. All data are expressed as the mean ± SD. n = 5/group