Fig. 9

Involvement of PI3K/Akt/NF-kB signaling in autophagy-induced angiogenesis 7 days after stroke. A KEGG pathway analysis of the genes in the apocynin group vs. the vehicle group compared with the vehicle group vs. the sham group. The top 10 pathways are shown. B Western blot analysis showing that inhibition of NOX2 by apocynin decreased the expression of PI3K/Akt/NF-kB signaling pathway proteins, including PI3K, p-PI3K, Akt, p-Akt, NF-κB p65 and p-NF-κB p65, as well as VEGF, and downregulated the autophagy proteins Beclin-1 and p62 in ischemic cortical tissue after 7 days of reperfusion. C–I Quantitative analysis of the protein levels of NOX2, Beclin-1, p62, p-PI3K, p-Akt, p-NF-κB p65 and VEGF in ischemic cortical tissue. *P < 0.05 vs. the sham group, ^&P < 0.05 vs. the vehicle group, ^#P < 0.05 vs. the APO group. All data are expressed as the mean ± SD. n = 5/group