Fig. 2

Analyses of CD45 subsets in MDMs and microglia at 7 days the post-ischemic brain. a Gating strategy for cell counts of CD11b⁺/CD45⁺/NK1.1⁻/Ly6G⁻ microglia and MDMs in R1’–R4’. b Cell counts for CD45^Low and CD45^High subsets within R1’–R4’. c Gating strategy for analysis of Ki-67 expression in R1’–R4’. d Cell counts of Ki67⁺/GFP⁻ cells in R1’ and R3’, as well as Ki67⁺/GFP⁺ cells in R2’ and R4’. e Counts of cells in d divided into CD45^Low and CD45^High expression. f Gating strategy for analysis of CD11c expression in R1’–R4’. g Cell counts of CD11c⁺/GFP⁻ cells in R1’ and R3’, as well as CD11c⁺/GFP⁺ cells in R2’ and R4’. h Counts of cells in g divided into CD45^Low and CD45^High expression. Statistical significance was assessed using one-way ANOVA with post hoc Bonferroni comparison test in d and g, whereas Student T-test was used in b, e, and h (with ^*, ^**, and ^***, respectively, indicating p < 0.05, 0.01, and 0.001)