Fig. 4.

Ex vivo phagocytosis assays in MDMs and microglia in the post-ischemic brain. For phagocytosis assays, the cells were isolated in the post-ischemic brain of asplenic mice that received GFP + splenocytes and bead^580/605 at 6th day after stroke. a Gating strategy for isolated cells containing GFP and /or bead^580/605 with subsets P1–P4 containing bead⁺ phagocytes. b Measures of phagocytic activity in GFP⁻ (P1, P3) and GFP⁺ (P2, P4) cells within the contralateral (P1, P2) and ipsilateral (P3, P4) hemispheres. Phagocytic activity was monitored by counts of cells containing bead^580/605 (counts), mean fluorescence intensity (MFI) of bead⁺ cells, and a phagocytic index (counts x MFI). c Cell count distribution of P1’–P4’ subsets, which contain CD45⁺/CD11b⁺/NK1.1⁻/Ly6G⁻ microglia and MDMs. d Measures of phagocytic activity for microglia (GFP⁻) and MDMs (GFP⁺) in the contralateral (P1’, P2’) and ipsilateral (P3’ P4’) hemispheres. Measures of statistical significance were determined by one-way ANOVA with post hoc Bonferroni comparison test (^**, ^***, and ^****, respectively, indicating p < 0.01, 0.001, 0.0001)