Fig. 6

Assessment of MDM phenotype conversion upon phagocytosis. a Gating strategy for measuring phagocytic activity of CD45⁺/CD11b⁺/NK1.1⁻/Ly6G⁻ microglia and MDMs isolated from the contra- and ipsi-lateral hemispheres in asplenic, stroked mice 1 day following an infusion of GFP⁺ splenocytes that were incubated for 4 h with bead^580/605. b Distribution of CD45⁺ subsets in GFP⁺ MDMs with or without bead^580/605, counts of CD45^Low and CD45^High with or without bead^580/605, and CD45^Low/CD45^High ratio of MDMs in cultures either with or without bead^580/605 (n = 5/group). Statistical significance was determined with Student T-test (^* and ^**, respectively, indicate p < 0.05 and 0.01). c In vivo phagocytic activity of CD45⁺/CD11b⁺/NK1.1⁻/Ly6G⁻ microglia and MDMs in asplenic mice receiving infusions of GFP⁺ splenocytes and bead^580/605 on the 6th day after stroke. Phagocytic activities were determined for CD45^Low (L) and CD45^High (H) cells (n = 8/group). Statistical significance was determined using Student T-test between CD45 subsets (^*, ^**, and ^****, respectively, indicate p < 0.05, 0.01, and 0.0001)