Fig. 5
From: Activation of peripheral TRPM8 mitigates ischemic stroke by topically applied menthol
Topically applied menthol (ME) to paws mitigates MCAO-induced astrocytosis and microgliosis. A, B Representative immunofluorescent photomicrographs of GFAP (A) and Iba1 (B) expression (red color), respectively, in the peri-infarct zone of brain segments from sham-operated mice, water- and menthol-treated Trpm8+/+ mice and Trpm8−/− mice on Day 2 following MCAO. The inset in low magnification is shown with a high magnification view in the below rows. DAPI staining is presented in blue. C, D Quantitative analysis of GFAP-positive astrogliosis and Iba1-positive microgliosis. †p < 0.05 vs sham; *p < 0.05 vs water-treated Trpm8+/+ mice; #p < 0.05 vs menthol-treated Trpm8+/+ mice; &p < 0.05 vs water-treated Trpm8−/− mice. E Density-plot graphs of FACS-sorted cells from sham-operated mice, water- and menthol-treated Trpm8+/+ mice on Day 1 following MCAO. Target cells (population 1, P1) are gated according to particle size and granularity and excluding cell debris and other irrelevant particles as much as possible. Q2 cell population, CD45high/CD11+ cells (monocytes/macrophages); Q4 cell population, CD45low/CD11+ cells (microglia). F Quantitative analysis of CD45high/CD11+ cell percentage in the brains. All data are expressed as the means ± S.E.M. In each bar, the symbols indicate the data of mice (n = 4–13) used in each study group. The scale bars represent 50 µm at 200X magnification